Affiliation(s)
1. Department of Chemical Engineering, USP, R. do Lago, São Paulo 250, 05508-080, Brazil
2. Department of Physical, Chemical and Geological Oceanography, USP, Praça do Oceanográfico 191, SP, Brazil
3. Department of Civil and Environmental Engineering, Rice University, 6100 Main St. Houston, Texas 77005, USA
ABSTRACT
The UV irradiation is used for removing
Antibiotic Resistant Bacteria (ARB) and Antibiotic Resistance Genes (ARG) from
wastewater treatment. Bacteriophages are viruses that infect within bacteria,
are recognized for bacterial control. The influence of some parameters in
quantification and performance influencing of pathogen demobilization could be
considered in disinfection of wastewater. The comparison of Polyvalent phage
(NE1) versus Coliphage (NE4) in suppressing a bacterium Escherichia coli (NDM-1: b-lactam-resistant) with UV irradiation was observed the efficacy in reduction of cells in
the disinfection and parameter process. The results with the effect of UV-C
irradiation on NDM-1 infected with 1 % of NE4 showed a decrease of cells from 8
x 106 to 2 x 105 in 60 min with UV-C dose. The NDM1 (E. coli) was infected with 1 % of NE4
(Polyvalent Phage) under magnetic stirring for 1 h, the cells count was 8 x 106.
After 1 h in UV-C exposure, the cells number reached 3 x 105. The
NDM1 that was exposed in 1 h of UV-C irradiation and then was infected with 1 %
of NE4. Cells counting were done 24 h after this procedure. These cells were
exposed in UV-C and showed a reduction in the number of cells from 1 x 108 to 4 x 105 after 60 min. The results indicate that bacteriophages
can mitigate bacteria species, and combined the conventional water disinfection
technologies that can support the microbial safety control strategies.
KEYWORDS
Antibiotic Resistant Bacteria (ARB), Antibiotic Resistance Genes (ARG),
wastewater treatment, disinfection, Escherichia
coli (E. coli).
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