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Time Dependent Motility of Stallion Semen during Cool Storage Centrifuged in Two Different Extenders
Christine Stroink-Pickering, Yuna Lee, Peyton Arnett and Kevin Halden Kline
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DOI:10.17265/2161-6256/2022.01.003
Department of Animal Sciences, University of Illinois at Urbana Champaign, 1207 West Gregory Drive, Urbana, IL 61801, USA
The aim of this study was to evaluate the maintenance of spermatozoa
motility over 72 h by two commercial semen extenders used to centrifuge semen before re-suspending the sperm
pellet in INRA 96®. Centrifuged and re-suspended semen samples were evaluated for percent motility
using four commercial Standard bred stallions
collected three times each during the spring and summer breeding season. Next
Generation Dr. Kenney’s® without antibiotic or
INRA 96® semen extenders were used to dilute semen samples in a 1:1
ratio prior to centrifugation at 750× g for 10 min. After
decanting the supernatant, the resulting sperm pellets were then re-suspended and
extended with INRA 96® to a final concentration of 50 million total
cells per millimeter and stored in Equine Express II stallion semen shipping
containers. Percent progressive motility was recorded at time points 0, 24, 48,
and 72 h. Analysis of variance between stallions, time, and extender type found
that there was no significant difference in percent motility at each of the
time points between the two extenders (p = 0.87) used for
centrifugation. Differences in percent motility over time and between stallions
were found (p < 0.001). The findings
suggest that the use of Next Generation Dr. Kenney’s® semen extender
during centrifugation and then re-suspending the sperm pellet in INRA 96® may
be more cost effective than using INRA 96® for both centrifugation and re-suspension of the
sperm pellet. This is due to the fact that the less expensive Next Generation
Dr. Kenney’s® supernatant is decanted and discarded after the centrifugation
step, instead of the much more expensive INRA 96®, yet does not
affect the quality of semen preservation and motility over time.
Semen, stallion, extender, centrifugation, motility.
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