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Article
Affiliation(s)

1. The Department of Food – Environment & Nursing, Dong Nai Technology University, Dong Nai 810000, Viet Nam
2. The Faculty of Science & Engineering, Hoa Sen University, Ho Chi Minh 700000, Viet Nam

ABSTRACT

Response surface methodology was used in this research to optimize the technical parameters of the extraction and conversion color compound in annatto seeds into norbixin. Parameters affected the extraction efficiency and norbixin recovery yield were solvent concentration, rate of solvent/seed, incubation time, and incubation temperature. The optimization results including: concentration of ethanol is 51.82°; concentration of NaOH is 0.52 M; ratio of solvent/seed is 7.1/1; incubation time is 33.12 minutes, incubation temperature is 58.6 °C, and the rate of 36% HCl/color solution is 0.5/5 (v/v). 

KEYWORDS

Achiote, Bixa orellana L., bixin, norbixin, extraction, conversion, food color. 

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