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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Principal Component Analysis (PCA) on Multivariate Data of Lard Analysis in Cooking Oil
Nor Aishah Mohd Salleh and Mohd Sukri Hassan
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DOI:10.17265/2159-5291/2015.07.005
1. Faculty of Science and Technology, Universiti Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia. 2. Institute of Halal Research & Management (IHRAM), Universiti Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
Discrimination of fatty acids (FAs) of lard in used cooking oil is important in halal determination. The aim of this study was to find the information related to the changes FAs of lard when frying in cooking oil. Quantitative analysis of FAs composition extracted from a series of experiments which involving frying cooking oil spiked with lard at three different parameters; concentration of spiked lard, heating temperatures and period of frying. The samples were analyzed using Gas Chromatography (GC) and Principal Components Analysis (PCA) technique. Multivariate data from chromatograms of FAs were standardized and computed using Unscrambler X10 into covariance matrix and eigenvectors correspond to Principal Components (PCs). Results have shown that the first and second PCs contribute to the FAs mapping which can be visualized by scores and loading plots to discriminate FAs of lard in used cooking oil.
Fatty acids, lard, gas chromatography, Principal Components Analysis
[2] A. Rohman and Y. B. Che Man, FTIR Spectroscopy combined with chemometrics for analysis of lard in the mixtures with body fats of lamb, cow, and chicken, Int. Food. Res J, 17 (2010) 519-527.
[3] D. Indrasti, Y. B. Che Man, S. Mustafa and D. M. Hashim. Lard detection based on fatty acids profile using comprehensive gas chromatography hyphenated with time-of-flight mass spectrometry, Food. Chem. 122 (2010) 1273-1277.
[4] J. M. N. Marikkar, H. M. Ghazali, Y. B. Che Man, T. S. G Peiris, and O. M. Lai, Distinguishing Lard from Other Animal Fats in Admixtures of Some Vegetable Oils Using Liquid Chromatographic Data Coupled with Multivariate Data Analysis, Food. Chem. 91 (1), (2005) 5-14.
[5] H. Abdi and L. J. Williams, Principal component analysis, Wiley Interdisciplinary Reviews: Computational Statistics, 2, 2010. pp. 433–459.
[6] B. Y. C. Christophe, “PCA: The Basic Building Block of Chemometrics”, Analytical Chemistry, 2012, pp. 2-10.
[7] H. E. Kim and G. Paul, “Principal Component Analysis: Concept, Geometrical Interpretation, Mathematical Background, Algorithms, History, Practice”, Chemical and Biochemical Data Analysis, 2, 2009, pp. 211–226.
[8] P. J. A. Shaw, Multivariate Statistics for the Environmental Sciences, London: Hodder Arnold, 4, 2003, pp. 73-82.
[9] E. G. Bligh and W. J. A. Dyer, Rapid method of total lipid extraction and purification, Can. J. Biochem. 37 (1959) 911-917.
[10] S. I. Cock and V. Rede, Laboratory Handbook for Oil and Fat Analysis. New York; Academic Press, Inc. Ltd. London, 1966.
[11] S. R. Eder, Über die bildung von artefakten bei der dampfung von speiseolen und-fetten. (Development of artifacts during processing of food oils and fats) fette-seifen-anstrichmittel, Fat-Sci. Tech. J. 84 (1982) 136-141.
[12] L. A. Medina-Juárez, N. Gámez-Meza, J. Ortega-García, J. A. Noriega Rodríguez and O. Angulo-Guerrero, Trans fatty acid composition and tocopherol content in vegetable oils produced in Mexico, J. of Am. Chem. Soc. 77 (2000) 721-724.
[13] F. A. Norris, Bailey's Industrial Oil and Fat Products, Wiley-Interscience, New York, 3, 4, 1985.