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Article
Oligosaccharides and Glycan Separation via Capillary Electrophoresis Coupled with Mass Spectroscopy
Author(s)
Khatia Merabishvili
Full-Text PDF XML 938 Views
DOI:10.17265/1934-7375/2021.01.003
Affiliation(s)
Department of Chemistry/Biochemistry, San Diego State University Georgia, Tbilisi 0108, Georgia
ABSTRACT
After 2014, capillary
electrophoresis (Fig. 1) has become an efficient method for carbohydrate evaluation. The methodology features a large
resolution capable of distinguishing carbohydrates by the ratio of charge to
scale. These primary features heavily focus on N-glycans, which seem to be
extremely relevant for biological therapeutics and biomarker researches.
Innovations in tools used during
molecular or structural recognition of N-glycan involve time-indexing of
migration and lectin profiling, also exoglycosidase along with mass
spectrometry. Techniques of capillary electrophoresis have been formed that are
able to separate glycans with the same sequence
of simple sugars similar to other instruments but it can detect different
positional isomers, and also specify
whether alpha or beta is linked to
monosaccharides comprising glycans.
Based on the significant accomplishments of capillary electrophoresis to
glycoscience from 2014 to the present, as addressed in this study,
groundbreaking, evolving techniques in the field are highlighted and the potential
direction of the technique is predicted.
KEYWORDS
N-glycan, exoglycosides, migration time index, APTS, CE-MS systems.
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