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Article
Study of Soil Microbiological Properties
Author(s)
Hamid Kheyrodin
Full-Text PDF XML 1288 Views
DOI:10.17265/1934-7375/2020.02.004
Affiliation(s)
Semnan University, Semnan, Iran
ABSTRACT
Two
strategies have been developed to improve DNA recovery in terms of yield,
purity and unbiased representation of the microbial diversity. However, amplification of DNA
from soil is often inhibited by co-purified contaminants. Furthermore, DNA is
also suitable for PCR amplification using various DNA targets. This review presents an overview of the
available methods to achieve this challenging objective. DNA was extracted from 100 g of soil using
direct lysis with glass beads and SDS followed by potassium acetate precipitation,
polyethylene glycol precipitation, phenol extraction and isopropanol
precipitation.
KEYWORDS
Purification, soil, DNA, RNA.
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