Affiliation(s)
1. Translational Research and Prototype Development Section, Malaysia Genome Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, Kajang 43000, Selangor, Malaysia
2. Structural Biology and Biophysics Facility Section, Malaysia Genome Institute, National Institutes of Biotechnology Malaysia, Jalan Bangi, Kajang 43000, Selangor, Malaysia
ABSTRACT
Despite advancement in food
analytical platforms and availability of CODEX standard, the fraud honey
relentlessly remains
as global issue. Even with stringent guidelines, controlling the incoming honey
product at ground level proved to be challenging and difficult to tackle. While
most analytical platforms are powerful enough to detect counterfeit honey products,
there is also a need to develop a rapid screening test to support field
regulatory activity. A chemical reagent developed based on biochemistry principle
has proved to be able to differentiate synthetic honey product from its raw
counterpart. The reaction required only three drops of honey product to react
with few drops of reagent mixtures which resulted in a change of color and
precipitation of proteins and other molecules within 2
min reaction. Test performed on raw honey samples
inclusive of several stingless bees and Apis species honey resulted in a
precipitation as compared to fake honey generated from a blend of white sugar,
golden syrup, vinegar, lime juice and plantain which resulted in clear organic
phase indicating missing natural honey biological matters. The difference
between raw honey and fake honey reaction with the reagents can be utilized for
real time on field screening activity before proceeding with authentication
using complex analytical platforms.
KEYWORDS
Raw honey, screening, fake honey, precipitation, rapid.
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