Affiliation(s)
1. College of Biotechnology, Misr University for Science and Technology (MUST), 6th October City 12566, Egypt
2. National Gene Bank (NGB), Agriculture Research Center (ARC), Giza 12619, Egypt
3. Bioinformatics Department, Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), Giza 12619, Egypt
4. Soils, Water and Environment Research Institute, Agriculture Research Center (ARC), Giza 12619, Egypt
ABSTRACT
Finding water resources for the desert plants is one of the important research areas since it enables saving water
resources. Bromus inermis plant was noticed to keep 5% of moisture in its rhizosphere zone; therefore, this study aimed
to identify this source of water. Thirteen endophytic
bacteria were isolated from the root of B. inermis and identified. Their
specific respiration rate was determined. Alcaligenes faecalis showed the highest specific respiration rate. It is a facultative
chemoautotrophic hydrogen-fixing bacterium that utilizes the hydrogen gas as
energy source and the water is produced as an end product. The source of
hydrogen gas for this bacterium is not only from air and soil gases but also
from the hydrogen-producing bacteria such as Enterobacter spp., which was among
the isolated bacteria. The hydrogenases synthesizing genes (HoxC, HypA and HypB) were detected in most of the
isolated bacteria and roots of four
wild plants, out from 18 wild plant samples,
epically the grains of the wild wheat plant. This result suggests that the hydrogen-fixing
and hydrogen-producing bacteria transfer from the root through the plant to
inhabit the grain/seeds. This can help the
grain/seeds to germinate in drought environment.
KEYWORDS
Desert plants, Alcaligenes spp., Enterobacter spp., hydrogenase, HoxC, HypA, HypB.
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