Affiliation(s)
1. Society of Veterinary Pharmaceutical and Biological Productions (Biopharma), B.P. 4569, Rabat, Morocco
2. Laboratory of Microbiology and Molecular Biology, Faculty of Sciences, University Mohammed V, 4 Ibn Battouta Ave. BP 1014 RP Rabat, Morocco
3. Institute of Agronomy and Veterinary Medicine Hassan II, B.P. 6202, Rabat, Morocco
ABSTRACT
Pasteurella species is considered the
principal pathogen of the respiratory tract. Mannheimia haemolytica and Pasteurella
multocida were investigated and typed from nasal swabs and tissues taken
from sheep, goat and cattle. Indeed, 41 lung and 121 nasal swabs samples were
collected from animals with respiratory diseases during 2015 to 2017 in six
different regions in Morocco. At first, a screening of Pasteurella species using the real time PCR (RT-PCR) was carried
out, then all isolated strains on agar blood were confirmed by PCR gel based
assay specific for M. haemolytica and P. multocida. Pathogenicity was evaluated in mice and
histopathological examination was done on some of lung tissue. The results
revealed that 34 samples of which 28 (55%) from nasal swabs and six (38%) from lungs
were positive for M. haemolytica and nine samples of which seven (14%) from nasal
swabs and two (13%) from lungs
were positive on P. multocida serogroup A. Seventy-two percent (72%)
isolates were highly
pathogenic to mice, which is in accordance
with the results obtained by histopathology examination. This is the first
report for widespread infections of Pasteurella (M. haemolytica & P. multocida) in ruminants in
Morocco. Therefore, measures including development of vaccines are highly
required to mitigate the impact of the bacteria in animals.
KEYWORDS
Isolation, identification, PCR, Mannheimia
haemolytica, Pasteurella multocida,
histopathology, Morocco.
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