Affiliation(s)
1. Faculty of Biotechnology, Vietnam National University of Agriculture, Trau Quy, Gia Lam 12406, Hanoi, Vietnam
2. Institute of Biotechnology, Vietnam Academy of Science and Technology, Nghia Tan, Cau Giay 11308, Hanoi, Vietnam
ABSTRACT
Glycinebetaine (GB), an osmotic substance, could improve some stress
tolerance in plants. CodA gene,
originating from bacteria, could translate choline oxidase which stimulates the
synthesis of GB in plants. To create lily lines resistant to heat, Belladonna
lily and Yelloween lily had been transferred CodA gene through Agrobacterium
tumefaciens. The bacteria harbored a binary vector carrying the hygromycin phosphotransferase, choline
oxidase (CodA) and intron-containing β-glucuronidase (Gus) genes were co-cultivated with lily bulb scales slides. The
result showed that most the bulb scales had developed into bulblets in a
regulator-free growth medium, while some expressed the hygromycin-resistance,
heat tolerance and Gus gene
expression. Among them, one line demonstrated primarily the transcription level
expression through reverse transcription polymerase chain reaction (RT-PCR). Moreover, they were
tested with the accumulation of GB which was evident that the transferred line
had four times of GB volumes higher than that of wild type. The original
evidence could open a right approach to enhance stress tolerance in lily
plants.
KEYWORDS
Agrobacterium tumefaciens, CodA gene,
genetic transformation, glycinebetaine, lily, stress tolerance in plants.
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