Production of Dried Lactobacillus plantarum HL-15 Culture for Inhibition Growth of Mycotoxin Producing Fungi

Lactobacillus plantarum HL-15 is able to inhibit the growth of mycotoxin producing fungi. To support the application of that culture, the research aimed to produce dried L. plantarum HL-15 and observe its stability during storage was conducted. Production of dried culture was started by fermentation of L. plantarum HL-15 then centrifuged to get the pellet. The pellet was mixed with filler (rice flour or tapioca) with a ratio 1:1 (pellet:filler (10%), v/v) then dried. The temperature of the inlet and outlet of the spray dryer used were 105 °C and 65 °C, respectively. Dried culture was packaged in aluminum foil and sealed then stored at 4 °C. Results showed that viable cells of dried inoculum with rice flour filler was 11.99 ± 0.01 log CFU/g and dried inoculum with tapioca filler was 11.90 ± 0.04 log CFU/g. Spray dried L. Plantarum HL-15 was proved being able to inhibit the growth of Aspergillus niger. During six months storage, there is a decrease in the viability of spray dried L. plantarum HL-15 either with rice flour or tapioca filler. During four months of storage, there was a decrease in the activity of inhibition of spray dried culture of L. plantarum HL-15 both with fillers of rice flour and tapioca to the growth of A. niger. L. plantarum HL-15 spray dried cultures could be stored at 4 °C for four months for tapioca and five months for rice flour as filler. L. plantarum HL-15 spray dried cultures could inhibit the growth of A. niger so it could be used as a culture for inhibiting the growth of mycotoxin producing fungi in food.