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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Carotenoids, Polyphenols and Antioxidant Activity Evaluation in Stone-Grinded Wheat Semolina
Author(s)
Pamela Vignolini1, Silvia Urciuoli1, Daniela Heimler2 and Annalisa Romani1
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DOI:10.17265/2328-7136/2018.06.006
Affiliation(s)
1. Laboratorio Phytolab, DiSIA, Dipartimento di Statistica, Informatica, Applicazioni“Giuseppe Parenti”, Università degli Studi di Firenze,Polo Scientifico e Tecnologico via U. Schiff, 6-Sesto Fiorentino (FI) 50019, Italy
2. PIN S.c.r.l.-Servizi Didattici e Scientifici per l’Università di Firenze, Piazza G. Ciardi, 25, Prato 59100, Italy
ABSTRACT
The functional quality of
stone grinded grain semolina, also related to the presence of secondary
antioxidant metabolites, was evaluated by HPLC/DAD (high performance liquid chromatography/diode array
detection) analysis and by in
vitro spectrophotometric techniques.
The total polyphenol content was determined with the
Folin-Ciocalteau spectrophotometric method, while carotenoid content evaluation
was performed by HPLC/DAD chromatographic analysis: the presence of lutein as
the main compound ranging between 75 and 78.5% of total carotenoids was pointed
out. The chromatographic analyses identified the presence of an indolacetic acid
derivative whose content changed between 0.097 and 0.108 mg/g.
KEYWORDS
HPLC/DAD, lutein, Folin-Ciocalteau, flavonoids.
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