Contact us
[email protected] | |
3275638434 | |
Paper Publishing WeChat |
Useful Links
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Physiological Maturity of Corn Seeds (Zea mays) by Tetrazolium Salt
Author(s)
Luiz Felipe Nicoleti Torrezan and Ana Dionisia da Luz Coelho Novembre
Full-Text PDF XML 598 Views
DOI:10.17265/2159-5828/2018.06.004
Affiliation(s)
The Department of Agriculture, University of Sao Paulo, Piracicaba 13418-900, Brazil
ABSTRACT
The purpose of this study was to evaluate the relationship of
tetrazolium salt testing to common indicators (milk line, black layer, dry matter) used to determine corn seed physiological maturity. The seeds used were
the hybrids Pioneer 4285 and Dow 2B587. These hybrids were harvested 40 days
after polinization (DAP), with 4-day intervals until 68 DAP.
The harvested seeds were evaluated for viability and vigor (germination test, accelerated aging
test and cold test). The parameters used to determine the physiological seed’s maturity were
the black layer, the milk line, the seed dry matter, seed water content and the
evaluation of seed tissues using the tetrazolium salt to evaluate the endosperm
cells. The germination of both materials was greater than 95% and there was no difference between harvest times.
Corn seed physiological maturity (PM) was identified at 56 DAP for P4285 (F2)
and between 48 and 56 DAP for 2B587
(F2). The maximum vigor was detected by accelerated aging tests and cold tests
before PM for the two genotypes. The activity of endosperm cells is related to
the other PM indicators (milk line, black layer, dry matter and water content).
The transport of nutrients from plant to the seed ceases at seed physiological
maturity. Use of tetrazolium salt allows identifying cell death of the basal
region and this parameter
corresponds to the others evaluated, thus demonstrating that the tetrazolium
salt is effective for corn seed physiological maturity characterization.
KEYWORDS
Formation, seed production, seed viability, black layer, milk line, corn seed vigor, corn seed germination.
Cite this paper
References