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Development and Validation of Proximal Chemistry Analytical Procedure in Semi-automated Equipment
Patricia Parra Cervantes M., Tonantzin Rios Rodríguez, Janet Edith Santamaría Víquez, Ramón Soto Vázquez and Joaquin Tlatempa López
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DOI:10.17265/2328-2150/2018.08.009
The proximal chemical
analysis (AQP) includes 5 fundamental tests, which are: determination of crude
protein, determination of crude fiber, determination of humidity, determination
of ashes and determination of fat. This last determination can be made in two
different ways, which will depend on the type of sample being treated, as well
as the amount of fat expected to be obtained in the food to be analyzed. For
foods with low amounts of fat the hydrolysis technique is used, which is
divided into 3 phases. All the methods before being taken to the daily
practice in a laboratory of food analysis either internal control, verification
or third authorized must be validated, in order to obtain consistent, robust
and reliable results. In those cases in which the method that will be
tested differs with the method that is reported in the literature, a comparison
of both methods should be made in order to ensure that both are compatible and
the results will be equally reliable. In
the validation, the acceptance parameters will be established for each one of
the tests that are carried out in it, while at the end of it the acceptance
criteria for the general method will be established. The
objective of this work was to carry out the development of analytical
methodology that was validatable in order to reduce analysis time by using
semi-automated equipment. In the case of semi-automated equipment, this
comparison of methods is carried out, as it was the case of the analysis of fat
with hydrolysis, which used a hydrolysis unit and the extraction equipment
using samples of finished food for animal consumption. The
results obtained in the validation using the traditional method correspond to a
CV less than 2%, while the results obtained using semi-automated equipment
correspond to a CV less than 2% for the case of fat determination with
hydrolysis.
Validation, analytical methods, semiautomatic, animal feed.
Cervantes, P. P., et al. 2018. “Development and Validation of Proximal Chemistry Analytical Procedure in Semi-automated Equipment.” Journal of Pharmacy and Pharmacology 6 (8): 782-789.