[email protected] | |
3275638434 | |
Paper Publishing WeChat |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Nor Syaidatul Akmal Mohd Yousof, Nikos Xynos, Evanthia Dina, Maizatul Hashima Omar, Choong Yew Keong, Mohd Isa Wasiman and Nektarios Aligiannis
Full-Text PDF XML 558 Views
DOI:10.17265/2328-2150/2018.08.001
The increasing demand for natural food
antioxidants has hastened
research to extract the biologically active substances from a variety of raw
materials. The consumption of anthocyanin-rich food promotes health by
contributing to cardio protection, lowering high blood pressure and by helping
to protect against cancer. In this study, centrifugal partition chromatography
(CPC) was studied for anthocyanin separation from Hibiscus
sabdariffa L. (HS). A simple elution and two displacement protocols were
implemented. The displacement modes were strong ion-exchange (SIX) and pH-zone refining (pHZR) method. Among the
three protocols, SIX CPC using trioctylmethylammonium chloride (Aliquat 336 ®) as a strong exchanger was the
most successful in purifying four anthocyanins from an ethanolic extract of HS,
identified as delphinidin-3-O-sambubioside
(DS), cyanidin-3-O-sambubioside (CS),
delphinidin-3-O-glucoside (DG) and
cyanidin-3-O-glucoside (CG). The
purification process was carried out on a gram scale using the ternary biphasic
system ethyl acetate:n-butanol:water (mobile organic phase:
4.0/4.6/1.4; aqueous stationary phase: 0.5/0.5/9.0) in the ascending mode. Aliquat 336® was used as the anion extractant in the aqueous stationary
phase and sodium iodide (NaI) as the cation displacer in the organic mobile
phase. From 1 g of crude HS
extract, 80 mg of DS, 40 mg of CS, 15 mg of DG, and 4 mg of CG were obtained
after 75 minutes. High performance liquid chromatography (HPLC-UV and LC-MS)
analysis revealed the identity and the purity of the isolated compounds: 88% for DS, 92% for CS, 93%
for DG and 70% for CG. This
new methodology for the isolation of anthocyanin mixtures successfully
increased the purity and efficiency especially for DG and CG, while maintaining
excellent recovery rate and being relatively low cost. The availability of high
purity anthocyanin mixtures will facilitate anthocyanin testing studies and
will widen the use of anthocyanins in the food, supplements and pharmaceutical
industries.
SIX, pHZR, CPC, Hibiscus sabdariffa, anthocyanins.
Xynos, N., et al. 2018. “Comparison of Standard Elution and Displacement Modes in Centrifugal Partition Chromatography for an Efficient Purification of Four Anthocyanins from Hibiscus sabdariffa L.” Journal of Pharmacy and Pharmacology 6 (8): 701-711.