Contact us
 |
[email protected] |
 |
3275638434 |
 |
 |
| Paper Publishing WeChat |
Useful Links
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Hasan Akay and Orhan Kurt
Full-Text PDF
XML 328 Views
DOI:10.17265/2161-6264/2018.02.003
Affiliation(s)
Field Crops Department, Faculty of Agriculture, Ondokuz Mayıs University, Samsun 55200, Turkey
ABSTRACT
In vitro callus induction and plant regeneration potentiality were studied from mature
embryo of three Indian rice (Oryza sativa L.) groups at Field Crops
Department, Agricultural Faculty, Ondokuz Mayıs
University, Samsun, Turkey. The study was done by
using callus induction MS medium having different concentration of four. The present research was conducted according to the design
of randomized blocks trial. A total of 696 calluses, 193 plants and 917 seeds
were obtained from Indica group; 2,110
calluses, 103 plants and 235 seeds were obtained from Japonica group; 1,243 calluses and 13
plants were obtained from Javanica group. With regard to number of calluses obtained
from each explant source, 52 calluses were obtained from whole-plant explants,
1,668
calluses from root explants, 629 calluses from shoot explants, 649 calluses
from the 1st node explants, 240 calluses from the 2nd node explants, 269
calluses from the 1st internode explants and 12 calluses from the 2nd internode
explants. With regard to number of plants obtained from each explant source, 27
plants were obtained from whole-plant explants, 195 plants from shoot explants,
43 plants from the 1st node explants, 40 plants from the 2nd node explants and four
plants from the 1st internode explants. With regard to number of seeds, 823
seeds were obtained from shoot explants and 329 seeds were obtained from the
2nd node explants. Germination rate of harvested seeds was over 90%. The establishment of this regeneration system is
essential for the development of a genetic transformation system for commercial
rice cultivars.
KEYWORDS
Rice, Oryza sativa L., callus induction, plant regeneration.
Cite this paper
References