Affiliation(s)
1. Department of Genetics, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, Poznań 61-614, Poland
2. Department of Plant Breeding and Seed Science, University of Agriculture in Kraków, Łobzowska 24, Kraków 31-140, Poland
ABSTRACT
The genetic variation of Italian ryegrass (Lolium multiflorum Lam.) and westerwolds ryegrass (L. westerwoldicum Breakw.) was investigated in
eight tetraploid cultivars originated from Poland. The 337 individual seedlings, cultivated in uniform
greenhouse conditions, were tested with horizontal gel electrophoresis
according to five enzyme systems: diaforase (DIA), superoxide dismutase (SOD),
glutamate oxalacetate transaminase (GOT), peroxidise (PX) and phosphogluco
isomerise (PGI), which were used to array
allelic diversity at five polymorphic loci. The estimation of genetic diversity
in 64 plants of the same cultivars was studied using inter
simple sequence repeats (ISSRs) as molecular marker. The genetic
variation described by ISSR suggests that the polymorphism detected appears to
be poorly informative at the taxonomic level. For statistical analysis, unweighted
pair group method with
arithmetic mean (UPGMA) based dendrograms and minimum spanning trees were
constructed using Nei’s distance and Jaccard’s similarity coefficient.
KEYWORDS
Lolium spp., tetraploid
cultivars, genetic structure, enzyme electrophoresis, inter simple sequence
repeats markers.
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