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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Agnes Kilonzo-Nthenge1,2, Samuel Nahashon2, Siqin Liu2, Ravneet Sandhu2 and KourtneyDaniels2
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DOI:10.17265/2159-5828/2018.01.001
Affiliation(s)
1. Department of Human Sciences, Tennessee State University, 3500 John A. Merritt Boulevard, Nashville, Tennessee 37209
2. Department of Agricultural and Environmental Sciences, Tennessee State University, 3500 John A. Merritt Blvd., Nashville, Tennessee 37209, USA
ABSTRACT
Antibiotics used for
agricultural purpose has contributed to the increased prevalence of
antibiotic-resistant bacteria. The goal of this study was to investigate the
prevalence and antimicrobial resistance of ESBL-producing E. coli in
small-scaled poultry farms and retail chicken. The cultured E. coli isolates were
subjected to phenotypic tests, susceptibility tests, and the polymerase chain
reaction for detection of blaCTX-M, blaSHV, and blaTEM genes. From 120 samples each of
chicken feces, retail chicken, soil and chicken feed, ESBL-producing E. coli isolates were detected in
75.9%, 63.6%, 39.2%, and 13.3% of the samples, respectively. Minimum inhibitory
concentration (MICs) values indicated that ESBL-producing E. coli were resistance to ampicillin (MIC
≥ 32 μg/mL), gentamicin (MIC
≥ 16 μg/mL), cefotaxime (MIC ≥ 4 μg/mL) and ceftriaxone (MIC
≥ 4 μg/mL), respectively. The total resistance for imipenem was
also observed at 1.0% (MIC ≥ 4 μg/mL) and none of the isolates were resistant to
ceftazidime (MIC ≥ 16 μg/mL). ESBL-producing E. coli from chicken feces and retail
chicken carried blaSHV gene at a rate of 6.8% and 5.7%,
respectively and blaCTX-M gene was also revealed at 2.9% in retail chicken.
Moreover, ESBL-producing E. coli isolated from soil harbored blaSHV and blaCTX-M genes at 5%.
None of the feed samples yielded ESBLs genes. Twenty three resistance patterns
were observed for multi-resistant ESBL-producing E.
coli. This study highlights the prevalence of multi-antimicrobial resistant
ESBL-producing E. coli in small-scaledpoultry
farms and retail chicken, hence the need to review poultry management practices
to minimize the occurrence.
KEYWORDS
E. coli, β-lactamases, poultry farms, retail chicken.
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