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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Nguyen Van Khanh1, Nguyen Thi Diem1, Le Thi Tuyet Nhan1, Phan Van Cu1, Tran Quang Khanh Van2 and Ngo Thi Hoan3
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DOI:10.17265/2161-6256/2017.10.008S
Affiliation(s)
1. Institute of Biotechnology, Hue University, Phu Thuong, Phu Vang, Thua Thien Hue 530000, Vietnam
2. Faculty of Fisheries, University of Agriculture and Forestry, Hue University, 102 Phung Hung Street, Hue 530000, Vietnam
3. Faculty of Fisheries, Ha Long University, 258 Bach Dang Street, Uong Bi City 207900, Quang Ninh, Vietnam
ABSTRACT
The main objective of the study was to determine
the effects of nutritional media and initial cell density on the growth and
development of Spirulina platensis. This study was
carried out at the Center for Incubation and Technology Transfer, Hue University from
May 2015 to August 2016. In the first experiment, S.
platensis was cultured in four different nutritional media, including Zarrouk, Spi-RIA3, Nisole and Thuoc
medium (Vietnamese).
Resulted showed that S. platensis grew
significantly in the Zarrouk medium rather than in other nutritional medium at
the same time, and gained the maximum density of 2,736.5 mg/L after 13 d inoculums, with specific growth rate
of 4.987% per day and the time to doubling population of
0.139 d. In the second experiment, S. platensis was
cultured in six different initial
cell densities: 100,
400, 700, 1,000, 1,300 and 1,600 mg/L, and the optimum
nutritional medium
was Zarrouk medium selected from
experiment one. Results showed at
the initial culture density of 400 mg/L, the highest biomass was gained with the maximum density of 3,071.2 mg/L after 21 d inoculums, with specific growth rate
of 9.15% per day and time to
double population of 0.076 d. S. platensis biomass
from culture in Zarrouk medium at initial cell density 400 mg/L obtained total protein
of 51.98%, total lipid of 1.75%, total carbohydrate of 0.75%, carotene of 0.36%
and total minerals of 5.2%, on a basis of dry
matter.
KEYWORDS
Spirulina platensis, nutrient medium, initial density, growth rates.
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