Affiliation(s)
1. School of Veterinary Medicine, Mongolian University of Life Sciences (MULS), Zaisan 17024, Ulaanbaatar, Mongolia
2. Research Institute of Animal Husbandry, Mongolian University of Life Sciences (MULS), Zaisan 17024, Ulaanbaatar, Mongolia
3. School of Engineering, Mongolian University of Life Sciences (MULS), Zaisan 17024, Ulaanbaatar, Mongolia
4. Institute of Veterinary Medicine, Mongolian University of Life Sciences (MULS), Zaisan 17024, Ulaanbaatar, Mongolia
ABSTRACT
In this study, mitochondrial 16S rRNA sequences of snow leopard, gray wolf, domestic horse and Bactrian camel inhabited or domesticated in
Mongolian territory were obtained by using polymerase chain reaction (PCR)
based on universal primers for 16S rRNA (F-5'-AACGAGCCTGGTGATA-3'
and R-5'-CTCCGGTCTGAACTCAGATCACGTA-3’). The 16S rRNA sequence was 1,048 bp to 1,086 bp in length, and each sequence was compared to other
related species (Felidae, Camelidae, Equidae and Canidae) by using NCBI Basic
Local Alignment Search Tool (BLAST). Results showed that sequences were highly
similar to sequences in GenBank database (93%-99%). Then phylogenetic analysis was performed based on about 1,100 bp sequence of
16S rRNA for Panthera uncia, Canis lupus, Equus caballus, Camelus bactrianus and other related species. The result revealed
that P. uncia and P. leo were sister species, C. bactrianus and C. ferus were more closely
related species, and wolf and dog were the almost similar species. This finding
could be important for designing species specific primers for PCR based
analysis of animal species identification and forensic veterinary medicine.
KEYWORDS
16S rRNA gene, sequence analysis, snow leopard, gray wolf, horse, Bactrian camel.
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