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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Charles Nelimor1, 2, Henry Yabbey Sintim1, 3, Alexander Wireko Kena1, 4 and Richard Akromah1
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DOI:10.17265/2161-6264/2017.02.001
Affiliation(s)
1. Department of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, PMB, Kumasi, Ghana
2. Department of Biosciences, Université Félix Houphouët-Boigny, BP 22, Abidjan, Cote D’lvoire
3. Department of Crop and Soil Sciences, Washington State University, 2606 West Pioneer, Puyallup, WA 98371, USA
4. Department of Agronomy, Horticulture & Plant Science, South Dakota State University, Brookings, SD 57007, USA
ABSTRACT
Kinetin is an important growth hormone used for in vitro propagation, but its dynamic and temporal effects on Dioscorea alata have not been thoroughly
evaluated. In this study, surface response
models were developed to better
elucidate the effects of kinetin on D.
alata propagated in vitro. Nodal
segments were obtained from Akaaba, an important D. alata cultivar in Ghana, and propagated in vitro under
five kinetin rates (0, 2.5, 5, 7.5 and 10 µM). The models were developed using
segmented multiple regression with time and kinetin as the predictors. The
effects on plant height, the number of leaves, shoots and roots were assessed
with three-dimensional figures for better observation of temporal trends. The
model fit was very good with normalized root mean squared error (NRMSE) = 0.1, R-squared = 0.83 and adjusted R-squared = 0.82, averaged across the
different growth parameters. Different kinetin levels elicited the maximum shoot, leaf and root
formation, as well as the growth rates over time. Moderate kinetin levels (2-4
µM) provided better growth at early culturing period. Higher kinetin levels
(5-10 µM) suppressed the
growth of the plantlets at early stages, but the plantlets recovered from the
stress and resumed normal growth thereafter. After 4-5 weeks, the growth rates of the moderate kinetin levels (2-4 µM)
declined much faster and were lower compared to the higher kinetin levels,
except plant height and the number of roots which were still higher at the moderate kinetin level even after eight weeks of culturing. Thus, kinetin requirements vary depending on the growth parameters of
interest.
KEYWORDS
Water yam, tissue culture, nodal explant, kinetin, surface response model.
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