Affiliation(s)
1. Department of Food Technology, Swiss German University, Tangerang, Banten 15339, Indonesia
2. Department of Food Technology, Pelita Harapan University, Karawaci, Tangerang, Banten 15810, Indonesia
ABSTRACT
Nowadays, consumers are demanding for minimally processed, additive-free,
high quality, fresh-like and microbiologically safe foods. However, as food
deterioration is the constant threat along the entire food chain, food
preservation remains as necessary today as in the past. In this paper, several citrus essential oils, including sweet
orange, lemon and lime essential oil applied directly in fresh orange juice
as a natural preservative, were analyzed for their antimicrobial activity. The
antimicrobial activity of each citrus essential
oil was assessed against Saccharomyces
cerevisiae, Lactobacillus plantarum and Aspergillus niger using broth
macrodilution. Among all citrus essential oils tested, sweet orange essential
oil with 0.10% concentration showed significant antimicrobial
activity against microorganisms tested. Hence, it was chosen to be applied in the fresh orange juice. Then, shelf life analysis
was conducted during 7 d of storage at 5 °C and during 2 d of storage at 25 °C using selected concentration of the selected citrus essential oil in the fresh orange juice. Based
on the results obtained, the addition of sweet orange essential oil with 0.10%
concentration was not able to prolong the shelf life of the fresh orange juice
according to the Indonesian National Standard (SNI). However, the
formulation of fresh orange juice itself was able to improve its shelf life
when compared to the shelf life of the commercial orange juice.
KEYWORDS
Fresh orange juice, spoilage, citrus essential
oils, preservative, antimicrobial.
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