Affiliation(s)
1. Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Daehak-ro 1, Gwanak-gu, Seoul 08826, Korea
2. Department of Veterinary Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 50877, Vietnam
3. Viral Infectious Disease Research Unit, Green Cross Veterinary Products, Yongin 17066, Republic of Korea
4. Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
5. Forensic Medicine Division, Daegu Institute, National Forensic Service, Chilgok 34054, Korea
ABSTRACT
Infectious agents causing
aborted fetus problems in domestic pigs were investigated in this study. More
than 10 different infectious agents were known to cause abortion in swine and
the major eight viruses among them were inspected. One hundred twelve samples
of aborted fetuses from nine provinces in South Korea were collected during
April to November, 2013 in this study for the diagnosis of infectious agents causing
abortions in pigs. Eight
major infection viruses were examined in this study mainly using various
diagnostic kits and reverse transcriptase
polymerase chain reaction (RT-PCR). Positive rate of
the detection differed from each viruses. In this study, the main focus was the
porcine reproductive and respiratory syndrome virus (PRRSV),
which took the
second large portion in the positive rate of detection, and then its ORF5 gene was
compared with modified live virus (MLV) vaccine strain
to figure out the influence of vaccine on disease. Between four positive samples’ sequence, two
of them were 99.9%-100%
similar to MLV vaccine strain and two other samples were 88.6%-92.7% similar. Similarity
rate of the sequences between the vaccine and virus from aborted fetuses are
very crucial, because it implies
that abortion in swine can be made due to the usage of vaccine not only by the
infection of field virus, and if MLV vaccine actually do have an impact on the
infection, usage of the vaccine should be reconsidered.
KEYWORDS
Abortion, infectious
agents, domestic pig, PRRSV, MLV vaccine.
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