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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Larissa Marila de Souza1,2, Natalia Mayumi Inada2, Sebastião Pratavieira2, Juliano José Corbi3, Cristina Kurachi2 and Vanderlei Salvador Bagnato2
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DOI:10.17265/1934-7391/2017.02.003
Affiliation(s)
1. Federal University of São Carlos, PPG Biotec, São Carlos 13565-905, São Paulo, Brazil
2. São Carlos Institute of Physics, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil
3. São Carlos School of Engineering, University of São Paulo, São Carlos 13560-970, São Paulo, Brazil
ABSTRACT
Dengue vector is responsible for millions of deaths every year and has
caused disastrous impacts on health systems. The continuous use of chemical
insecticides, such as carbamates, pyrethroids and organophosphates generates
resistant populations of the mosquito, therefore, new control methods must be
investigated. The joint action of the population and guidelines for preventing
the reproduction of the mosquito associated with the use of photoactivatable
insecticides can be the alternative for the control of epidemiological
outbreaks in affected regions. In this study, the photo-larvicidal activity of Photogem®(PG), a derivative of
hematoporphyrin, was investigated against 2nd–early 3rd instar of Aedes
aegypti larvae (Diptera: Culicidae) under different lighting conditions
(artificial lighting system and sunlight). The dynamics of PG accumulation was
characterized by CLSM (confocal laser scanning microscopy) and total time PG
eliminationin solution was investigated by ultraviolet-visible
spectrophotometry. The maximum photo-activity of PG was observed in 0.5 h under
sunlight exposure which achieved 100% larval mortality. Fluorescence images
showed a uniform distribution of PG along the digestive tract. PG remained
stable in the sunlight for 48 h and in an artificial lighting system for longer
periods, therefore, it can be used for the control of Aedes aegypti larvae as a new alternative to chemical insecticides.
The method is considered environmentally friendly due to its rapid degradation
in the presence of light. Further studies are required, so that the potential
of the technique can be explored in real breeding places.
KEYWORDS
Photoactivatable larvicide, PG, hematoporphyrin derivative, Aedes aegypti, natural lighting, artificial lighting.
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