Affiliation(s)
1. Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
2. School of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom
ABSTRACT
The goal of this
study was to evaluate the water retention value (WRV) of a test solid substrate and a fungal cell in solid state
fermentation (SSF). WRV is the ratio
of the weight of water retained after centrifugation under specific conditions
by a wet sample to the oven dry weight of the same sample. SSF refers to the
microbial fermentation, which takes place in the absence or near absence of
free water, thus being close to the natural environment. Many factors are
involved in a successful SSF process. In
addition to biological parameters, the SSF process is also dependent on
physical factors such as WRV. A centrifugal
technique has been modified and applied to the evaluation of WRV. Wheat bran, soybean hulls and
rapeseed meal were used as model substrate. Aspergillus
awamori and Aspergillus oryzae were used as model microorganism. Results revealed that the ability of wheat bran to retain water in the
solid substrate is 56% higher than that of soybean hulls and rapeseed meal. In the term of fungal cell, the ability of A. oryzae to retain water in the cells was higher (73% higher) than
that of A. awamori. In addition, through oven method moisture content loss
from A. awamori is 46% higher than that from A. oryzae during drying process. Nevertheless,
it can be seen that A. oryzae is able to retain water content about 5 times higher than A. awamori. Through this results, we
found that WRV varies depending on solid substrates and microorganisms.
This initial information can be beneficial in the SSF process to be carried
out.
KEYWORDS
Water retention value
(WRV), solid state fermentation,
solid substrates, Aspergillus awamori, Aspergillus oryzae.
Cite this paper
References