Affiliation(s)
1. Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, Raichur 584101, Karnataka, India
2. Department of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584101, Karnataka, India
3. Department of Agronomy, University of Agricultural Sciences, Raichur 584101, Karnataka, India
ABSTRACT
The study has been initiated with an aim to utilize cytoplasmic
male sterile (CMS) associated gene
fragment marker to understand the marker flow in segregating population and
nature of dominance of the marker. And further it was aimed to understand the
best pollination time for the maximum fruit set and to economise the chilli hybrid
seed production based on CMS lines. Hence, two CMS based high yielding hybrids, which were found to
be much more potential than that of the non CMS based hybrids, have been
identified. The CMS gene was dissected from one of the high yielding hybrid.
The marker was successfully amplified in A line, F1 and F2 population with a polymerase chain reaction (PCR) product of 600
bp. The seedlings were transplanted at the ratio of
2:1, 2:2 and 3:2 sterile:fertile for natural pollination and 2:1 sterile:fertile for
artificial pollination. The percent of fruit set was calculated without emasculation (CMS
line) and pollination, and with emasculation and pollination, respectively.
The maximum fruit set of 95.24% per plant was recorded when artificial crossing attempted
between 10:00 am and 11:00 am using male
sterile lines. While, 40% fruit set was observed in emasculation and
pollination system. The maximum numbers of fruit set (351 and 75) were registered in JNA1 and ACA1 male sterile lines, respectively, thorough artificial
pollination. However, the maximum numbers of fruit set (20.24 and 14.74)
were recorded in JNA1 and ACA1, respectively, by natural pollination. Pollinating more number of
flowers and fruit set success was recorded using male sterile lines than that
of the bisexual plant in chilli.
KEYWORDS
Cytoplasmic male sterility,
molecular marker, standardization, emasculation, pollination, chilli.
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