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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Author(s)
Mahmoud Ben Othman1, Mohamed Neffati3 and Hiroko Isoda1, 2
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DOI:10.17265/2161-6256/2017.02.003
Affiliation(s)
1. Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan
2. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-8572, Japan
3. Range Ecology Laboratory, Arid Land Institute of Medenine, Medenine 4119, Tunisia
ABSTRACT
Many antioxidant rich
phytochemical extracts are attracting
interest as stress adaptogens. In this regard, several plants are
used traditionally and clinically for the management of neurologic disorders. Here, the
anti-stress properties of the ethanol
extract of medicinal plant Cymbopogon schoenanthus (CSEE), which grows
wild in Southern Tunisia, were investigated, as well as the effects of some of
the extracted active compounds on H2O2-induced
cytotoxicity, overproduction of reactive oxygen species (ROS)and adenosine triphosphate (ATP) depletion in
human neuroblastoma SH-SY5Y cells. Whereas fluorescence intensity due to DCFH-DA (a marker of ROS production) in H2O2-treated
SH-SY5Y cells was greater than that in untreated control cells, pretreatment with CSEE and its active compounds
ameliorated H2O2-induced ROS production. Moreover, H2O2 at 150 µM significantly decreased intracellular ATP levels in SH-SY5Y cells, and pretreatment
with CSEE and its active compounds buffered this effect. These in vitro results demonstrate the potential of CSEE to protect against
stress-associated disorders.
KEYWORDS
ATP levels, H2O2, medicinal plant, reactive oxygen species, stress.
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