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Article
Affiliation(s)

1. Plant Extraction Laboratory, Department of Pharmaceutical Biotechnologies, National Institute of Chemical-Pharmaceutical Research and Development, Bucharest 031299, Romania
2. Microbiological Laboratory, Department of Pharmacology, National Institute of Chemical-Pharmaceutical Research and Development, Bucharest 031299, Romania

ABSTRACT

This study aimed to evaluate polyphenols content, antioxidant and antimicrobial activities of ethanol extract from rock rose (Helianthemum nummularium (L.) Mill.). Plant material has been harvested in July from Romanian Carpathian Mountains. The ethanolic extract (70%, v/v) was prepared from the aerial part of rock rose species. High performance thin layer chromatography (HPTLC) analysis of ethanol extract and subsequently hydrolysed sample indicated that quercetin glycosides were the major polyphenolic compounds, and kaempferol derivates, chlorogenic and gallic phenylcarboxylic acids are also being present in the polar extracts. Chemiluminescence assay in luminol/H2O2 system indicated very high antioxidant activity of the ethanolic type of extract (IC50 = 1.27 μg/mL), while microbiological studies (cylinder method in plates) indicated certain antimicrobial activity (measuring from 12.5 mm to 21.5 mm of the diameter of the inhibition zone) of the propylene glycol (20%, v/v) standardized extract (5 mg gallic acid equivalents/mL sample) against several standard bacterial strains, Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028 and Salmonella enterritidis ATCC 13076, respectively. Therefore, based on the augmented antioxidant effect of the rock rose polar extracts, there can be found numerous applications in the pharmaceutical industry, but also in non-pharmaceutical fields, for example in cosmetic and hygiene products areas, due to certain antimicrobial properties.

KEYWORDS

Helianthemum nummularium (L.) Mill., polyphenols content, antioxidant and antimicrobial activity.

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