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Article
Author(s)
Shaif Mohammed Kasem Saleh1, Amer Mohammed Mussaed1 and Fadhl Mohammed Al-Hariri2
Full-Text PDF XML 869 Views
DOI:10.17265/2161-6264/2016.02.009
Affiliation(s)
1. Department of Chemistry, Faculty of Science & Education, University of Aden, Aden, Yemen
2. National Quality Control Laboratory, Aden, Yemen
ABSTRACT
Honey is being used as a
pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics
than ever before, so honey testing has become essential to maintain its
healthful characteristics and protect public health. Tetracyclines
(TCs) are
used for the treatment or prevention of American and European foul broad in bee
colonies which are caused especially by two species of bacteria—Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine
the
tetracycline
residues in different types of local and imported honey. The reversed-phase
high performance liquid chromatography (HPLC) with ultraviolet detection
(UV)
was
used. Tetracycline and oxytetracycline were extracted from honey with 0.01
M
sodium succinate buffer. Of the examined samples,
31.25% and 12.50% had these oxytetracycline and tetracycline residues,
respectively.
It
was observed that all the samples studied have been reported
residues of tetracycline and oxytetracycline more than international
standards of
maximum residue limit (MRLs).
KEYWORDS
Honey, antibiotic residues, tetracycline, oxytetracycline, HPLC, Yemen.
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