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Mastitis Pathogens Isolated from Samples of Milk in Dairy Cows and Their Resistance against Antimicrobial Agents



Author(s)

František Zigo¹, Milan Vasiľ¹, Juraj Elečko¹, Martina Zigová² and Zuzana Farkašová¹

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DOI:10.17265/2159-5828/2017.02.007

1. Department of Animal Husbandry, University of Veterinary Medicine and Pharmacy, Košice 040 01, Slovak Republic
2. Department of Pharmacology, Pavol Josef Šafarik University, Košice 040 01, Slovak Republic

The objective of this study was to evaluate the effectiveness of different antibiotics against mastitis causing microorganisms during first phase of lactation in herd of 320 Holstein cows in east of Slovakia. Milk samples from quarters were cultured and identified bacteria were subjected to antimicrobial susceptibility test by disc diffusion method to a large number of antibiotics. The results revealed higher sensitivity against tetradelta (100% of Strep. Agalactiae and Strep. uberis, Coagulase Negative Staphylococci), (94.4% of Stap. aureus) with highest number of bacterial isolates, followed by cefalexin + kanamycin amoxicillin plus clavulanat acid and ceftiotur (100% of Strep. agalactiae and Strep. uberis). Resistance was observed against penicillin (22.2% of Stap. aureus) amoxicillin (22.2% of Stap. aureus and 10.5% of Strep. uberis) and streptomycin (22.2% of Stap. aureus and 52.7% of Strep. uberis).

Mastitis, dairy cows, resistance, Staphylococcus aureus, Streptococcus agalactiae.