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Mioara Negoita, Enuta Iorga, Alina Adascalului, Luminita Catana, Nastasia Belc, Andreea Stan, Dragos Efstatiade and Hassan Y. Aboul-Enein
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DOI:10.17265/2162-5298/2016.04.002
The acrylamide level of some commercial bread assortments have been evaluated. The influence of several flour types on the content of acrylamide in 14 samples of bread, purchased from 5 different suppliers from the market in Bucharest, Romania was studied. For all bread assortments which have been analyzed for acrylamide, the International Commission of Illumination (CIELab) parameters, L*, a* and b* have been also measured in an attempt to correlate the colour of the final product with its acrylamide content. Determination of acrylamide in bread samples was performed by Gas Chromatography coupled with tandem Mass Spectrometry (GC-MS/MS), using isotope dillution calibration after derivatization (bromination). The results revealed that the acrylamide level vary between bread assortments of different brands and bread assortments of same brand, but different lots. In the case of commercial bread samples, the use of whole wheat flours lead to formation of higher quantity in acrylamide (165.53 µg/kg), compared to the use of white wheat flours (28.02 µg/kg).
Acrylamide, flour, bread, GC/MS/MS, CIELab parameters