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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Article
Highly Sensitive Filter-Less Fluorescence Detection Method Using an Avalanche Photodiode
Author(s)
Masahiro Akiyama1, Kazuya Miyazawa1 and Kazuaki Sawada2
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DOI:10.17265/1934-8975/2016.04.008
Affiliation(s)
1. Department of Electrical and Electronic Engineering, National Institute of Technology, Nagano College, Nagano 381-8550, Japan
2. Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Aichi 441-8580, Japan
ABSTRACT
Herein
we report a highly sensitive filter-less fluorescence detection method using an
APD (avalanche photodiode). Experimental measurements using the proposed
APD-based highly sensitive fluorescence detection method exhibits the sensing
capability to detect an excitation light and a fluorescence light without band
pass filter or grating. The principle of this APD-based highly fluorescence
detection method is used the varying multiplication ratio that is decided by
wavelength. The wavelength controls running distance of photo-excited carrier
by absorption coefficients, and this element decide multiplication ratio on
fixed high electrical field. In fluorescence detection, they use two types of
light: excitation light and fluorescence light. These lights have different wavelengths
and make different multiplication ratio as well. Thus this method can separate
two types of light easily by using multiplication ratios of APD without band
pass filters/gratings. In this experiment, the excitation light is LED (light
emitting diode) and fluorescence light
occurs from FITC (fluorescein isothiocyanate) with ethanol. The FITC
concentration changes from 0.1 mmol/L to 10 mmol/L. In this measurement circuit, we employ APD
(S2385), power supply voltage, and pico ampere current meter. As a result,
these lights are correctly separated by using multiplication ratio with
calculation at every concentration FITCs.
KEYWORDS
Avalanche photodiode, filter-less, fluorescence.
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